학술논문
Phylogenomics and genetic analysis of solvent-producing Clostridium species.
Document Type
Academic Journal
Author
Jensen RO; LanzaTech Inc, Skokie, IL, USA.; Schulz F; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Roux S; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Klingeman DM; Oak Ridge National Laboratory, Oak Ridge, TN, USA.; Mitchell WP; LanzaTech Inc, Skokie, IL, USA.; Udwary D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Moraïs S; Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.; Reynoso V; LanzaTech Inc, Skokie, IL, USA.; Winkler J; LanzaTech Inc, Skokie, IL, USA.; Nagaraju S; LanzaTech Inc, Skokie, IL, USA.; De Tissera S; LanzaTech Inc, Skokie, IL, USA.; Shapiro N; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Ivanova N; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Reddy TBK; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Mizrahi I; Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.; Utturkar SM; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA.; Bayer EA; Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.; Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, 7610001, Israel.; Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; University of California Merced, Life and Environmental Sciences, Merced, CA, USA.; Mouncey NJ; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Jewett MC; Department of Bioengineering, Stanford University, Stanford, CA, USA.; Simpson SD; LanzaTech Inc, Skokie, IL, USA.; Köpke M; LanzaTech Inc, Skokie, IL, USA.; Jones DT; Department of Microbiology, University of Otago, Dunedin, New Zealand. butanolman@gmail.com.; Brown SD; LanzaTech Inc, Skokie, IL, USA. steve.brown@lanzatech.com.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101640192 Publication Model: Electronic Cited Medium: Internet ISSN: 2052-4463 (Electronic) Linking ISSN: 20524463 NLM ISO Abbreviation: Sci Data Subsets: MEDLINE
Subject
Language
English
Abstract
The genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes), whose members can encode useful complex traits such as solvent production, gas-fermentation, and lignocellulose breakdown. We describe 270 genome sequences of solventogenic clostridia from a comprehensive industrial strain collection assembled by Professor David Jones that includes 194 C. beijerinckii, 57 C. saccharobutylicum, 4 C. saccharoperbutylacetonicum, 5 C. butyricum, 7 C. acetobutylicum, and 3 C. tetanomorphum genomes. We report methods, analyses and characterization for phylogeny, key attributes, core biosynthetic genes, secondary metabolites, plasmids, prophage/CRISPR diversity, cellulosomes and quorum sensing for the 6 species. The expanded genomic data described here will facilitate engineering of solvent-producing clostridia as well as non-model microorganisms with innately desirable traits. Sequences could be applied in conventional platform biocatalysts such as yeast or Escherichia coli for enhanced chemical production. Recently, gene sequences from this collection were used to engineer Clostridium autoethanogenum, a gas-fermenting autotrophic acetogen, for continuous acetone or isopropanol production, as well as butanol, butanoic acid, hexanol and hexanoic acid production.
(© 2024. The Author(s).)
(© 2024. The Author(s).)