학술논문
In vitro evaluation of injectable Tideglusib-loaded hyaluronic acid hydrogels incorporated with Rg1-loaded chitosan microspheres for vital pulp regeneration.
Document Type
Academic Journal
Author
Atila D; Department of Engineering Sciences, Middle East Technical University, Ankara 06800, Turkey; Institute of Biomedical Engineering & Nanomedicine (IBEN), National Health Research Institutes, Miaoli 35053, Taiwan.; Chen CY; Department of Biomedical Sciences & Engineering, National Central University, Taoyuan City 320317, Taiwan.; Lin CP; School of Dentistry and Graduate Institute of Clinical Dentistry, National Taiwan University and Hospital, Taipei 106216, Taiwan.; Lee YL; School of Dentistry and Graduate Institute of Clinical Dentistry, National Taiwan University and Hospital, Taipei 106216, Taiwan.; Hasirci V; Department of Medical Engineering, Acibadem Mehmet Ali Aydinlar University, Istanbul 34758, Turkey; BIOMATEN, CoE in Biomaterials & Tissue Engineering, Middle East Technical University, Ankara 06800, Turkey.; Tezcaner A; Department of Engineering Sciences, Middle East Technical University, Ankara 06800, Turkey; BIOMATEN, CoE in Biomaterials & Tissue Engineering, Middle East Technical University, Ankara 06800, Turkey. Electronic address: tezcaner@metu.edu.tr.; Lin FH; Institute of Biomedical Engineering & Nanomedicine (IBEN), National Health Research Institutes, Miaoli 35053, Taiwan; Institute of Biomedical Engineering, College of Medicine & College of Engineering, National Taiwan University, Taipei 106216, Taiwan. Electronic address: double@ntu.edu.tw.
Source
Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE
Subject
Language
English
Abstract
Injectable systems receive attention in endodontics due to the complicated and irregular anatomical structure of root canals. Here, injectable Tideglusib (Td)-loaded hyaluronic acid hydrogels (HAH) incorporated with Rg1-loaded chitosan microspheres (CSM) were developed for vital pulp regeneration, providing release of Td and Rg1 to trigger odontoblastic differentiation of human dental pulp stem cells (DPSC) by Td and vascularization of pulp by Rg1. The optimal concentrations were determined as 90 nM and 50 μg/mL for Td and Rg1, and loaded in HA and CSM in HAH, respectively. Odontogenic (COL1A1, ALP, OCN, Axin-2, DSPP, and DMP1) and angiogenic (VEGFA, VEGFR2, and eNOS) differentiation of DPSC cultured in the presence of hydrogels was shown at gene expression level. Our results suggest that our injectable hydrogel formulation has potential to improve strategies for vital pulp regeneration. In vivo evaluations are needed to test the feasibility and potential of these hydrogels for vital pulp regeneration.
(Copyright © 2021. Published by Elsevier Ltd.)
(Copyright © 2021. Published by Elsevier Ltd.)