학술논문
Characterization and optimization of bioluminescent bacterial cells immobilization process in calcium alginate hydrogel tablets.
Document Type
Academic Journal
Author
Harpaz D; Department of Postharvest Science, Institute of Postharvest and Food Science, Volcani Center, Agricultural Research Organization, Rishon LeZion 7505101, Israel.; Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 76100, Israel.; Zoabi K; Department of Postharvest Science, Institute of Postharvest and Food Science, Volcani Center, Agricultural Research Organization, Rishon LeZion 7505101, Israel.; Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 76100, Israel.; Eltzov E; Department of Postharvest Science, Institute of Postharvest and Food Science, Volcani Center, Agricultural Research Organization, Rishon LeZion 7505101, Israel.
Source
Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
Subject
Language
English
Abstract
Aims: Whole-cell biosensors are increasingly utilized in various applications. These platforms integrate cells with a signal measurement device. One of the main challenges in the development of such platforms is the immobilization matrix that is used to keep the cells stable, which also affects the portability of the device. In this study, a portable and simple immobilization of bioluminescent bacterial cells in calcium alginate hydrogel was examined.
Methods and Results: The effects of several physical parameters were investigated (e.g. calcium alginate solution volume, drying, incubation time, mixing procedure, bacterial concentration, and tablet location within the cylinder). An alginate solution volume of 3 ml was preferred as well as the addition of 400 μl solution after the 15 min of compressing step and before the polymerization step. Also, a stirring mixing mode is favored over vortexing due to the creation of better homogenized tablets, as well as a bacterial concentration of 0.15 OD600nm that produced a high light response while maintaining a lower variance. Lastly, the findings showed a significantly higher response [induction factor (IF)] in the tablets using the optimized immobilization protocol (IF = 8.814) than the old one (IF = 1.979).
Conclusions: To conclude, bacterial cells immobilization in calcium alginate tablets provides improved sensitivity and storability.
(© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Methods and Results: The effects of several physical parameters were investigated (e.g. calcium alginate solution volume, drying, incubation time, mixing procedure, bacterial concentration, and tablet location within the cylinder). An alginate solution volume of 3 ml was preferred as well as the addition of 400 μl solution after the 15 min of compressing step and before the polymerization step. Also, a stirring mixing mode is favored over vortexing due to the creation of better homogenized tablets, as well as a bacterial concentration of 0.15 OD600nm that produced a high light response while maintaining a lower variance. Lastly, the findings showed a significantly higher response [induction factor (IF)] in the tablets using the optimized immobilization protocol (IF = 8.814) than the old one (IF = 1.979).
Conclusions: To conclude, bacterial cells immobilization in calcium alginate tablets provides improved sensitivity and storability.
(© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)