학술논문
Bioelectrochemical Nitrogen fixation (e-BNF): Electro-stimulation of enriched biofilm communities drives autotrophic nitrogen and carbon fixation.
Document Type
Academic Journal
Author
Rago L; e-BioCenter, Department of Environmental Science and Policy, Università di Milano, Via Celoria 2, 20133 Milano, Italy.; Zecchin S; e-BioCenter, Department of Food Environmental and Nutritional Science, Università di Milano, Via Mangiagalli 25, 20133 Milano, Italy.; Villa F; e-BioCenter, Department of Food Environmental and Nutritional Science, Università di Milano, Via Mangiagalli 25, 20133 Milano, Italy.; Goglio A; e-BioCenter, Department of Environmental Science and Policy, Università di Milano, Via Celoria 2, 20133 Milano, Italy.; Corsini A; e-BioCenter, Department of Food Environmental and Nutritional Science, Università di Milano, Via Mangiagalli 25, 20133 Milano, Italy.; Cavalca L; e-BioCenter, Department of Food Environmental and Nutritional Science, Università di Milano, Via Mangiagalli 25, 20133 Milano, Italy.; Schievano A; e-BioCenter, Department of Environmental Science and Policy, Università di Milano, Via Celoria 2, 20133 Milano, Italy. Electronic address: andrea.schievano@unimi.it.
Source
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100953583 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-562X (Electronic) Linking ISSN: 15675394 NLM ISO Abbreviation: Bioelectrochemistry Subsets: MEDLINE
Subject
Language
English
Abstract
A new approach to microbial electrosynthesis is proposed, aimed at producing whole biomass from N 2 and inorganic carbon, by electrostimulation of complex microbial communities. On a carbon-based conductor under constant polarization (-0.7 V vs SHE), an electroactive biofilm was enriched with autotrophic nitrogen fixing microorganims and led to biomass synthesis at higher amounts (up to 18 fold), as compared to controls kept at open circuit (OC). After 110 days, the electron transfer had increased by 30-fold, as compared to abiotic conditions. Metagenomics evidenced Nif genes associated with autotrophs (both Archaea and Bacteria) only in polarized biofilms, but not in OC. With this first proof of concept experiment, we propose to call this promising field 'bioelectrochemical nitrogen fixation' (e-BNF): a possible way to 'power' biological nitrogen fixation, organic carbon storage and soil fertility against desertification, and possibly a new tool to study the development of early prokaryotic life in extreme environments.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
(Copyright © 2018 Elsevier B.V. All rights reserved.)