학술논문
Failure of glucose-binding lectins Con A and Lentil Lectin to identify glycation of haemoglobin.
Document Type
Academic Journal
Author
Frantzen F; Axis Nord AS, Morkved, Norway.; Sundrehagen E
Source
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0043535 Publication Model: Print Cited Medium: Print ISSN: 0008-6215 (Print) Linking ISSN: 00086215 NLM ISO Abbreviation: Carbohydr Res Subsets: MEDLINE
Subject
Language
English
ISSN
0008-6215
Abstract
We have studied the interaction of Concanavalin A and Lentil Lectin with glycohaemoglobin by a nephelometric lectin-glycogen/dextran precipitation system and monitored the inhibitory effect of glycohaemoglobin on the precipitation. Although inhibitory effects were clearly demonstrated using simple sugars and transferrin, no effect was observed by glycohaemoglobin in relevant concentrations. This is compared to affinity chromatography, binding studies using gel filtration and electrophoresis, and affinity studies using Concanavalin A immobilised on magnetisable polymer particles. Lack of interaction between glycohaemoglobin and lectins is discussed in view of steric constraints and reduced availability of the glycated residues and the stereochemical form of the glycated 1-amino-1-deoxy-fructosyl residues in glycohaemoglobin.