학술논문
Biphasic alcoholysis coupled with high-speed countercurrent chromatography for high performance on separating phorbol from Croton tiglium Linn extracts.
Document Type
Academic Journal
Author
Fan JP; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.; Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Nanchang University, Nanchang, P. R. China.; He BT; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.; Gao Y; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.; Xie CF; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.; Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Nanchang University, Nanchang, P. R. China.; Chen HP; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.; Peng HL; Department of Chemical Engineering, Nanchang University, Nanchang, P. R. China.
Source
Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101088554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-9314 (Electronic) Linking ISSN: 16159306 NLM ISO Abbreviation: J Sep Sci Subsets: MEDLINE
Subject
Language
English
Abstract
Phorbol is a tetracyclic diterpenoid found in Euphorbia tirucalli, Croton tiglium, and Rehmannia glutinosa, and is nuclear of various phorbol esters. The rapid obtaining of phorbol with high purity highly contributes to its application, such as synthesizing phorbol esters with designable side chains and particular therapeutic efficacy. This study introduced a biphasic alcoholysis method for obtaining phorbol from croton oil by using polarity imparity organic solvents in both phases and established a high-speed countercurrent chromatography method for simultaneous separation and purification of phorbol. The optimized operation conditions of biphasic alcoholysis were a reaction time of 91 min, a temperature of 14°C, and a croton oil-methanol ratio of 1:30 (g:ml). The phorbol during the biphasic alcoholysis was 3.2-fold higher in content than that obtained in conventional monophasic alcoholysis. The optimized high-speed countercurrent chromatography method was using the ethyl acetate/n-butyl alcohol/water at 4.7:0.3:5 (v:v:v) with Na 2 SO 4 at 0.36 g/10 ml as the solvent system, using the mobile phase flow rate of 2 ml/min, the revolution of 800 r/min, under which the retention of the stationary phase was achieved at 72.83%. The crystallized phorbol following high-speed countercurrent chromatography was obtained as high purity of 94%.
(© 2023 Wiley-VCH GmbH.)
(© 2023 Wiley-VCH GmbH.)