학술논문
Characterization of the microbiome and volatile compounds in anal gland secretions from domestic cats (Felis catus) using metagenomics and metabolomics.
Document Type
Academic Journal
Author
Rojas CA; Genome Center, University of California-Davis, Davis, CA, USA. conniearojas6@gmail.com.; Department of Evolution and Ecology, University of California-Davis, Davis, CA, USA. conniearojas6@gmail.com.; Marks SL; Department of Medicine and Epidemiology, University of California-Davis, School of Veterinary Medicine, Davis, CA, USA.; Borras E; Department of Mechanical and Aerospace Engineering, University of California-Davis, Davis, CA, USA.; UC Davis Lung Center, University of California-Davis, Davis, CA, USA.; Lesea H; Department of Microbiology and Molecular Genetics, University of California-Davis, Davis, CA, USA.; McCartney MM; Department of Mechanical and Aerospace Engineering, University of California-Davis, Davis, CA, USA.; UC Davis Lung Center, University of California-Davis, Davis, CA, USA.; VA Northern California Health Care System, Mather, CA, USA.; Coil DA; Genome Center, University of California-Davis, Davis, CA, USA.; Davis CE; Department of Mechanical and Aerospace Engineering, University of California-Davis, Davis, CA, USA.; UC Davis Lung Center, University of California-Davis, Davis, CA, USA.; VA Northern California Health Care System, Mather, CA, USA.; Eisen JA; Genome Center, University of California-Davis, Davis, CA, USA.; Department of Evolution and Ecology, University of California-Davis, Davis, CA, USA.; Department of Medical Microbiology and Immunology, University of California-Davis, Davis, CA, USA.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Many mammals rely on volatile organic chemical compounds (VOCs) produced by bacteria for their communication and behavior, though little is known about the exact molecular mechanisms or bacterial species that are responsible. We used metagenomic sequencing, mass-spectrometry based metabolomics, and culturing to profile the microbial and volatile chemical constituents of anal gland secretions in twenty-three domestic cats (Felis catus), in attempts to identify organisms potentially involved in host odor production. We found that the anal gland microbiome was dominated by bacteria in the genera Corynebacterium, Bacteroides, Proteus, Lactobacillus, and Streptococcus, and showed striking variation among individual cats. Microbiome profiles also varied with host age and obesity. Metabolites such as fatty-acids, ketones, aldehydes and alcohols were detected in glandular secretions. Overall, microbiome and metabolome profiles were modestly correlated (r = 0.17), indicating that a relationship exists between the bacteria in the gland and the metabolites produced in the gland. Functional analyses revealed the presence of genes predicted to code for enzymes involved in VOC metabolism such as dehydrogenases, reductases, and decarboxylases. From metagenomic data, we generated 85 high-quality metagenome assembled genomes (MAGs). Of importance were four MAGs classified as Corynebacterium frankenforstense, Proteus mirabilis, Lactobacillus johnsonii, and Bacteroides fragilis. They represent strong candidates for further investigation of the mechanisms of volatile synthesis and scent production in the mammalian anal gland.
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)