학술논문
Thermoresponsive keratin-methylcellulose self-healing injectable hydrogel accelerating full-thickness wound healing by promoting rapid epithelialization.
Document Type
Academic Journal
Author
Dixit K; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India; Immunology and Inflammation Research Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Bora H; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Chakrabarti R; Cardiovascular biology lab, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Saha B; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Dogra N; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Biswas S; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Sengupta TK; Midnapore Medical College, West Midnapore, West Bengal 743368, India.; Kaushal M; Department of Chemical Engineering, IIT Kharagpur, West Bengal 721302, India.; Rana S; Department of Basic Science and Humanities, University of Engineering and Management, New Town, Action Area-III, Kolkata 700160, India.; Mukherjee G; Immunology and Inflammation Research Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India.; Dhara S; Biomaterial Tissue Engineering Laboratory, School of Medical Science and Technology, IIT Kharagpur, 721302, West Bengal, India. Electronic address: sdhara@smst.iitkgp.ac.in.
Source
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
Subject
Language
English
Abstract
Chronic wounds suffer from impaired healing due to microbial attack and poor vascular growth. Thermoresponsive hydrogels gained attention in wound dressing owing to their gelation at physiological temperature enabling them to take the shape of asymmetric wounds. The present study delineates the development of thermoresponsive hydrogel (MCK), from hair-derived keratin (K) and methylcellulose (MC) in the presence of sodium sulfate. The gelation temperature (T g ) of this hydrogel is in the range of 30 °C to 33 °C. Protein-polymer interaction leading to thermoreversible sol-gel transition involved in MCK blends has been analyzed and confirmed by FTIR, XRD, and thermal studies. Keratin, has introduced antioxidant properties to the hydrogel imparted cytocompatibility towards human dermal fibroblasts (HDFs) as evidenced by both MTT and live dead assays. In vitro wound healing assessment has been shown by enhanced migration of HDFs in the presence of MCK hydrogel compared to the control. Also, CAM assay and CD31 expression by the Wistar rat model has shown increased blood vessel branching after the implantation of MCK hydrogel. Further, in vivo study, demonstrated MCK efficacy of hydrogel in accelerating full-thickness wounds with minimal scarring in Wistar rats, re-epithelialization, and reinstatement of the epidermal-dermal junction thereby exhibiting clinical relevance for chronic wounds.
Competing Interests: Declaration of competing interest There are no conflicts to declare.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of competing interest There are no conflicts to declare.
(Copyright © 2024 Elsevier B.V. All rights reserved.)