학술논문
Inter- and intraobserver agreement in whole-slide digital ThinPrep samples of low-grade squamous lesions of the cervix uteri with known high-risk HPV status: A multicentric international study.
Document Type
Academic Journal
Author
Kholová I; Tampere University, Faculty of Medicine and Health Technology, Tampere, Finland.; Pathology, Fimlab Laboratories, Tampere, Finland.; Negri G; Pathology Unit, Central Hospital Bolzano, Italy.; Nasioutziki M; Faculty of Medicine, Aristotle University of Thessaloniki and Molecular Cytopathology Department, Hippokration General Hospital, Thessaloniki, Greece.; Ventura L; Department of Statistical Sciences, University of Padova, Padova, Italy.; Capitanio A; Department of Pathology, University Hospital Linköping, Linköping, Sweden.; Bongiovanni M; Synlab Pathology, Lausanne, Switzerland.; Cross PA; South of Tyne Pathology Department, Queen Elizabeth Hospital, Gateshead, England.; Bourgain C; Imelda Hospital, Bonheiden, Belgium.; Edvardsson H; Department of Pathology and Cytology, Karolinska University Laboratory, Karolinska Hospital Huddinge, Stockholm, Sweden.; Granados R; University Hospital de Getafe, Madrid, Spain.; Lipiński A; Department of Pathology and Oncological Cytology, Wroclaw Medical University, Wroclaw, Poland.; Obermann EC; University Basel, Basel, Switzerland.; Pinamonti M; University Hospital Cattinara, Trieste, Italy.; Sidlova H; St. Elisabeth Cancer Institute Hospital, Bratislava, Slovak Republic.; Strojan Fležar M; Faculty of Medicine, University of Ljubljana, Institute of Pathology, Ljubljana, Slovenia.; van Kemenade FJ; Erasmus MC University Medical Center, Department of Pathology, Rotterdam, the Netherlands.; Vrdoljak-Mozetic D; Department of Pathology and Cytology, Clinical Hospital Centre Rijeka, Rijeka, Croatia.; Fassina A; Department of Medicine (DIMED), Surgical Pathology & Cytopathology Unit, University of Padova, Padova, Italy.; Cochand-Priollet B; Cochin Hospital, University of Paris, Paris, France.
Source
Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 101499453 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6638 (Electronic) Linking ISSN: 1934662X NLM ISO Abbreviation: Cancer Cytopathol Subsets: MEDLINE
Subject
Language
English
Abstract
Background: High-risk human papilloma virus (HR HPV) testing and liquid-based cytology are used for primary cervical screening. Digital cytology, based on whole-slide scanned samples, is a promising technique for teaching and diagnostic purposes. The aim of our study was to evaluate the interobserver and intraobserver variation in low-grade squamous lesions, HR HPV status bias, and the use of whole-slide scanned digital cervical cytology slides.
Methods: Fifteen expert cytopathologists evaluated 71 digitalized ThinPrep slides (31 atypical squamous cells of undetermined significance [ASC-US], 21 negative for intraepithelial lesion or malignancy, and 19 low-grade squamous intraepithelial lesion cases). HR HPV data were accessible only in the second round.
Results: In interobserver analysis, Kendall's coefficient of concordance was 0.52 in the first round and 0.58 in the second round. Fleiss' kappa values were 0.29 in the first round and 0.31 in the second round. In the ASC-US category, Fleiss kappa increased from 0.19 to 0.22 in the second round and the increase was even higher expressed by Kendall's coefficient: from 0.42 to 0.52. In intraobserver analysis, personal scores were higher in the second round.
Conclusions: The interobserver and intraobserver variability in low-grade squamous lesions was within fair agreement values in the present study, in line with previous works. The comparison of two rounds showed that expert cytopathologists are generally unbiased by the knowledge of HR HPV data, but that being informed of the HR HPV status leads to a better agreement. Stain quality and back discomfort were highlighted as factors affecting digital cytopathology use.
(© 2022 The Authors. Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.)
Methods: Fifteen expert cytopathologists evaluated 71 digitalized ThinPrep slides (31 atypical squamous cells of undetermined significance [ASC-US], 21 negative for intraepithelial lesion or malignancy, and 19 low-grade squamous intraepithelial lesion cases). HR HPV data were accessible only in the second round.
Results: In interobserver analysis, Kendall's coefficient of concordance was 0.52 in the first round and 0.58 in the second round. Fleiss' kappa values were 0.29 in the first round and 0.31 in the second round. In the ASC-US category, Fleiss kappa increased from 0.19 to 0.22 in the second round and the increase was even higher expressed by Kendall's coefficient: from 0.42 to 0.52. In intraobserver analysis, personal scores were higher in the second round.
Conclusions: The interobserver and intraobserver variability in low-grade squamous lesions was within fair agreement values in the present study, in line with previous works. The comparison of two rounds showed that expert cytopathologists are generally unbiased by the knowledge of HR HPV data, but that being informed of the HR HPV status leads to a better agreement. Stain quality and back discomfort were highlighted as factors affecting digital cytopathology use.
(© 2022 The Authors. Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.)