학술논문
Origanum majorana L. polyphenols: in vivo antiepileptic effect, in silico evaluation of their bioavailability, and interaction with the NMDA receptor.
Document Type
Academic Journal
Author
Amaghnouje A; Laboratory of Biotechnology, Health, Agrofood and Environment (LBEAS), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco.; Chebaibi M; Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez, Morocco.; Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.; Aldossari SM; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.; Ghneim HK; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.; Amrati FE; Laboratory of Cell Biology and Molecular Genetics (LBCGM), Department of Biology, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco.; Es-Safi I; Laboratory of Biotechnology, Health, Agrofood and Environment (LBEAS), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco.; Di Cristo F; National Research Council, Research Institute on Terrestrial Ecosystems (IRET), Porano, Italy.; Calarco A; National Research Council, Research Institute on Terrestrial Ecosystems (IRET), Porano, Italy.; Achour S; Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez, Morocco.; Carta F; NEUROFARBA Department, Sezione di Scienze Farmaceutiche e Nutraceutiche, University of Florence, Florence, Italy.; Al-Sheikh YA; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.; Aboul-Soud MAM; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.; Bousta D; Laboratory of Biotechnology, Health, Agrofood and Environment (LBEAS), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Source
Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101627988 Publication Model: eCollection Cited Medium: Print ISSN: 2296-2646 (Print) Linking ISSN: 22962646 NLM ISO Abbreviation: Front Chem Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2296-2646
Abstract
Introduction: Epilepsy is a chronic brain disease characterized by repeated seizures and caused by excessive glutamate receptor activation. Many plants are traditionally used in the treatment of this disease. This study aimed to evaluate the bioavailability of a polyphenolic extract obtained from Origanum majorana L. (OMP) leaves, as well as its antiepileptic activity and its potential mechanism of action. Methods: We have developed and validated a simple, rapid, and accurate stability-indicating reversed-phase liquid chromatographic method for the simultaneous determination of caffeine and quercetin in rat plasma. The OMP antiepileptic effect was evaluated with pilocarpine-induced seizures, and a docking method was used to determine the possible interaction between caffeic acid and quercetin with the N-methyl-D-aspartate (NMDA) receptor. Results and Discussion: Both compounds tested showed low bioavailability in unchanged form. However, the tested extract showed an anticonvulsant effect due to the considerably delayed onset of seizures in the pilocarpine model at a dose of 100 mg/kg. The molecular docking proved a high-affinity interaction between the caffeic acid and quercetin with the NMDA receptor. Taken together, OLP polyphenols demonstrated good antiepileptic activity, probably due to the interaction of quercetin, caffeic acid, or their metabolites with the NMDA receptor.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Amaghnouje, Chebaibi, Aldossari, Ghneim, Amrati, Es-Safi, Di Cristo, Calarco, Achour, Carta, Al-Sheikh, Aboul-Soud and Bousta.)
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Amaghnouje, Chebaibi, Aldossari, Ghneim, Amrati, Es-Safi, Di Cristo, Calarco, Achour, Carta, Al-Sheikh, Aboul-Soud and Bousta.)