학술논문
Multiscale label-free imaging of myelin in human brain tissue with polarization-sensitive optical coherence tomography and birefringence microscopy.
Document Type
Academic Journal
Author
Blanke N; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.; Chang S; Department of Electrical & Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA 02215, USA.; Novoseltseva A; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.; Wang H; Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129, USA.; Boas DA; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.; Bigio IJ; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.; Department of Electrical & Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA 02215, USA.
Source
Publisher: Optica Publishing Group Country of Publication: United States NLM ID: 101540630 Publication Model: eCollection Cited Medium: Print ISSN: 2156-7085 (Print) Linking ISSN: 21567085 NLM ISO Abbreviation: Biomed Opt Express Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2156-7085
Abstract
The combination of polarization-sensitive optical coherence tomography (PS-OCT) and birefringence microscopy (BRM) enables multiscale assessment of myelinated axons in postmortem brain tissue, and these tools are promising for the study of brain connectivity and organization. We demonstrate label-free imaging of myelin structure across the mesoscopic and microscopic spatial scales by performing serial-sectioning PS-OCT of a block of human brain tissue and periodically sampling thin sections for high-resolution imaging with BRM. In co-registered birefringence parameter maps, we observe good correspondence and demonstrate that BRM enables detailed validation of myelin (hence, axonal) organization, thus complementing the volumetric information content of PS-OCT.
Competing Interests: The authors declare no conflicts of interest.
(© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)
Competing Interests: The authors declare no conflicts of interest.
(© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)