학술논문
S100A12 inhibits fibroblast migration via the receptor for advanced glycation end products and p38 MAPK signaling.
Document Type
Academic Journal
Author
Tanaka N; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Ikari J; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan. junikari@chiba-u.jp.; Anazawa R; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Suzuki M; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Katsumata Y; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Shimada A; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Suzuki E; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Matsuura Y; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Kawata N; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Tada Y; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.; Tatsumi K; Department of Respirology (B2), Graduate School of Medicine, Chiba University, 1-8-1, Inohana, Chuo Ward, Chiba City, 260-8670, Japan.
Source
Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE
Subject
Language
English
Abstract
The migration of lung fibroblasts plays a pivotal role in wound repair and fibrotic processes in the lung. Although the receptor for advanced glycation end products (RAGE) has been implicated in the pathogenesis of lung diseases, its role in lung fibroblast migration is unclear. The current study examined the effect of three different RAGE ligands, namely, high mobility group box 1 (HMGB1), S100A12, and N-epsilon-(carboxymethyl) lysine (CML), on human fibronectin-directed human fetal lung fibroblast (HFL-1) migration. HMGB1 augmented, whereas S100A12 inhibited, HFL-1 migration in a concentration-dependent manner. CML did not affect HFL-1 migration. The effect of HMGB1 was not through RAGE. However, the effect of S100A12 was mediated by RAGE, but not Toll-like receptor 4. S100A12 did not exert a chemoattractant effect, but inhibited HFL-1 chemotaxis and/or chemokinesis. Moreover, S100A12 mediated HFL-1 migration through p38 mitogen-activated protein kinase (MAPK) but not through nuclear factor-kappa B, protein kinase A, phosphatase and tensin homolog deleted on chromosome 10, or cyclooxygenase. In addition, western blot analysis showed that S100A12 augmented p38 MAPK activity in the presence of human fibronectin. In conclusion, S100A12 inhibits lung fibroblast migration via RAGE-p38 MAPK signaling. This pathway could represent a therapeutic target for pulmonary conditions characterized by abnormal tissue repair and remodeling.