학술논문
Phenotypes for general behavior, activity, and body temperature in 3q29 deletion model mice.
Document Type
Academic Journal
Author
Mori D; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan. d-mori@med.nagoya-u.ac.jp.; Brain and Mind Research Center, Nagoya University, Nagoya, Japan. d-mori@med.nagoya-u.ac.jp.; Department of Pathophysiology of Mental Disorders, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan. d-mori@med.nagoya-u.ac.jp.; Ikeda R; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Sawahata M; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.; Yamaguchi S; Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.; Kodama A; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Hirao T; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Arioka Y; Department of Pathophysiology of Mental Disorders, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.; Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya, Japan.; Okumura H; Department of Pathophysiology of Mental Disorders, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Inami C; Department of Pathophysiology of Mental Disorders, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.; Suzuki T; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Hayashi Y; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Kato H; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Nawa Y; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Miyata S; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Kimura H; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Kushima I; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Medical Genomics Center, Nagoya University Hospital, Nagoya, Japan.; Aleksic B; Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan.; Mizoguchi H; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Nagai T; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Division of Behavioral Neuropharmacology, International Center for Brain Science (ICBS), Fujita Health University, Toyoake, Japan.; Nakazawa T; Laboratory of Molecular Biology, Department of Bioscience, Graduate School of Life Sciences, Tokyo University of Agriculture, Tokyo, Japan.; Hashimoto R; Department of Pathology of Mental Diseases, National Institute of Mental Health, National Center of Neurology and Psychiatry, Tokyo, Japan.; Kaibuchi K; Division of Cell Biology, International Center for Brain Science, Fujita Health University, 1-98 Dengakugakubo, Kusukake-cho, Toyoake, Aichi, Japan.; Kume K; Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.; Yamada K; Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University, Graduate School of Medicine, Nagoya, Japan.; Ozaki N; Department of Pathophysiology of Mental Disorders, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.; Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan.
Source
Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101562664 Publication Model: Electronic Cited Medium: Internet ISSN: 2158-3188 (Electronic) Linking ISSN: 21583188 NLM ISO Abbreviation: Transl Psychiatry Subsets: MEDLINE
Subject
Language
English
Abstract
Whole genome analysis has identified rare copy number variations (CNV) that are strongly involved in the pathogenesis of psychiatric disorders, and 3q29 deletion has been found to have the largest effect size. The 3q29 deletion mice model (3q29-del mice) has been established as a good pathological model for schizophrenia based on phenotypic analysis; however, circadian rhythm and sleep, which are also closely related to neuropsychiatric disorders, have not been investigated. In this study, our aims were to reevaluate the pathogenesis of 3q29-del by recreating model mice and analyzing their behavior and to identify novel new insights into the temporal activity and temperature fluctuations of the mouse model using a recently developed small implantable accelerometer chip, Nano-tag. We generated 3q29-del mice using genome editing technology and reevaluated common behavioral phenotypes. We next implanted Nano-tag in the abdominal cavity of mice for continuous measurements of long-time activity and body temperature. Our model mice exhibited weight loss similar to that of other mice reported previously. A general behavioral battery test in the model mice revealed phenotypes similar to those observed in mouse models of schizophrenia, including increased rearing frequency. Intraperitoneal implantation of Nano-tag, a miniature acceleration sensor, resulted in hypersensitive and rapid increases in the activity and body temperature of 3q29-del mice upon switching to lights-off condition. Similar to the 3q29-del mice reported previously, these mice are a promising model animals for schizophrenia. Successive quantitative analysis may provide results that could help in treating sleep disorders closely associated with neuropsychiatric disorders.
(© 2024. The Author(s).)
(© 2024. The Author(s).)