학술논문
The effects of exercise and mitochondrial transplantation alone or in combination against Doxorubicin-induced skeletal muscle atrophy.
Document Type
Academic Journal
Author
Kubat GB; Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences, Ankara, Turkey. gokhanburcin.kubat@sbu.edu.tr.; Gulhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey. gokhanburcin.kubat@sbu.edu.tr.; Ulger O; Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences, Ankara, Turkey.; Gulhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey.; Atalay O; Department of Physiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.; Fatsa T; Gulhane Health Sciences Institute, University of Health Sciences, Ankara, Turkey.; Turkel I; Department of Exercise and Sport Sciences, Faculty of Sport Sciences, Hacettepe University, Ankara, Turkey.; Ozerklig B; Department of Exercise and Sport Sciences, Faculty of Sport Sciences, Hacettepe University, Ankara, Turkey.; Celik E; Department of Pathology, Gulhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey.; Ozenc E; Department of Pathology, Gulhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey.; Simsek G; Department of Pathology, Gulhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey.; Tuncer M; Department of Physiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Source
Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 8006298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2657 (Electronic) Linking ISSN: 01424319 NLM ISO Abbreviation: J Muscle Res Cell Motil Subsets: MEDLINE
Subject
Language
English
Abstract
Doxorubicin (DOX) is a chemotherapy drug used to treat various types of cancer, but it is associated with significant side effects such as skeletal muscle atrophy. Exercise has been found to prevent skeletal muscle atrophy through the modulation of mitochondrial pathways. Mitochondrial transplantation (MT) may mitigate toxicity, neurological disorders, kidney and liver injury, and skeletal muscle atrophy. The objective of this study was to evaluate the effects of MT, exercise, and MT with exercise on DOX-induced skeletal muscle atrophy. Male Sprague Dawley rats were randomly assigned to the following groups: control, DOX, MT with DOX, exercise with DOX, and exercise with MT and DOX. A 10-day treadmill running exercise and MT (6.5 µg/100 µL) to tibialis anterior (TA) muscle were administered prior to a single injection of DOX (20 mg/kg). Our data showed that exercise and MT with exercise led to an increase in cross-sectional area of the TA muscle. Exercise, MT and MT with exercise reduced inflammation and maintained mitochondrial enzyme activity. Additionally, exercise and MT have been shown to regulate mitochondrial fusion/fission. Our findings revealed that exercise and MT with exercise prevented oxidative damage. Furthermore, MT and MT with exercise decreased apoptosis and MT with exercise triggered mitochondrial biogenesis. These findings demonstrate the importance of exercise in the prevention of skeletal muscle atrophy and emphasize the significant benefits of MT with exercise. To the best of our knowledge, this is the first study to demonstrate the therapeutic effects of MT with exercise in DOX-induced skeletal muscle atrophy.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)