학술논문
In situ identification and G4-PPI-His-Mal-dendrimer-induced reduction of early-stage amyloid aggregates in Alzheimer's disease transgenic mice using synchrotron-based infrared imaging.
Document Type
Academic Journal
Author
Benseny-Cases N; ALBA Synchrotron Light Source, Carrer de la Llum 2-26, 08290, Cerdanyola del Vallès, Catalonia, Spain. nbenseny@gmail.com.; Álvarez-Marimon E; Unitat de Biofísica i Centre d'Estudis en Biofísica, Departament de Bioquímica i de Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain.; Aso E; Biomedical Network Research Center of Neurodegenerative Diseases (CIBERNED), University of Barcelona, 08907, Hospitalet de Llobregat, Catalonia, Spain.; Carmona M; Biomedical Network Research Center of Neurodegenerative Diseases (CIBERNED), University of Barcelona, 08907, Hospitalet de Llobregat, Catalonia, Spain.; Klementieva O; Medical Microspectroscopy, Department of Experimental Medical Science, Lund University, 22180, Lund, Sweden.; Appelhans D; Leibniz Institute of Polymer Research, Dresden, Hohe Strase 6, 01069, Dresden, Free State of Saxony, Germany.; Ferrer I; Biomedical Network Research Center of Neurodegenerative Diseases (CIBERNED), University of Barcelona, 08907, Hospitalet de Llobregat, Catalonia, Spain.; Cladera J; Unitat de Biofísica i Centre d'Estudis en Biofísica, Departament de Bioquímica i de Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain. josep.cladera@uab.cat.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Amyloid plaques composed of Aβ amyloid peptides and neurofibrillary tangles are a pathological hallmark of Alzheimer Disease. In situ identification of early-stage amyloid aggregates in Alzheimer's disease is relevant for their importance as potential targets for effective drugs. Synchrotron-based infrared imaging is here used to identify early-stage oligomeric/granular aggregated amyloid species in situ in the brain of APP/PS1 transgenic mice for the first time. Also, APP/PS1 mice show fibrillary aggregates at 6 and 12 months. A significant decreased burden of early-stage aggregates and fibrillary aggregates is obtained following treatment with poly(propylene imine) dendrimers with histidine-maltose shell (a neurodegenerative protector) in 6-month-old APP/PS1 mice, thus demonstrating their putative therapeutic properties of in AD models. Identification, localization, and characterization using infrared imaging of these non-fibrillary species in the cerebral cortex at early stages of AD progression in transgenic mice point to their relevance as putative pharmacological targets. No less important, early detection of these structures may be useful in the search for markers for non-invasive diagnostic techniques.
(© 2021. The Author(s).)
(© 2021. The Author(s).)