부산대학교 도서관

G raptopetalum paraguayense was shown to exhibit antioxidant, hepatoprotective and anti-hepatocellular carcinoma activities in a recent study. In this work, fractions of the G . paraguayense aqueous solution were extracted using n-hexane, ethyl acetate ( EF) and n-butanol, and its antioxidative and anti-glycative activities were evaluated. The EF extract exhibited the highest antioxidative activity, potently inhibited the formation of advanced glycation end products ( AGEs) and reduced glycation-induced protein oxidation. Additionally, the EF extract protected Hep G2 cells against AGE-induced DNA damage. Five major components were isolated and identified in the EF fraction by semi-preparative high-performance liquid chromatography, liquid chromatography-tandem mass spectroscopy and nuclear magnetic resonance: gallic acid, isoquercitrin-6-(3-hydroxy-3-methylglutarate), astragalin-6-(3-hydroxy-3-methylglutarate), isoquercitrin-2-acetyl-6-(3-hydroxy-3-methylglutarate) and astragalin-2-acetyl-6-(3-hydroxy-3-methylglutarate). G . paraguayense exhibited antioxidant and anti-glycation activities, likely because of the presence of gallic acid. The findings indicate the potential for using G . paraguayense for development of novel treatments for diabetes. Practical Applications Herbal treatments for hyperlipidemia are relatively cheap and locally available. The results indicated that the extracts of G raptopetalum paraguayense may be developed as functional foods for antidiabetic treatment mediated by attenuations of advanced glycation end product oxidation. [ABSTRACT FROM AUTHOR]