학술논문

"Inject-Mix-React-Separate-and-Quantitate" (IMReSQ) Method for Screening Enzyme Inhibitors.
Document Type
Article
Source
Journal of the American Chemical Society. 9/10/2008, Vol. 130 Issue 36, p11862-11863. 2p. 1 Diagram, 1 Chart, 1 Graph.
Subject
*ENZYME inhibitors
*PROTEINS
*ORGANIC compounds
*BIOSYNTHESIS
*CATALYSTS
Language
ISSN
0002-7863
Abstract
The article introduces a method called inject-mix-react-separate-and-quantitate, for screening enzyme inhibitors which is applicable to regulatory enzymes and consume only nanoliter volumes of the reactant solutions. The reaction mixture is incubated to form the enzymatic product. The methods for screening enzyme inhibitors are homogeneous and separation-based.