학술논문
A Simple Voltage Controlled Enzymatic Nanoreactor Produced in the Tip of a Nanopipet.
Document Type
Article
Source
Subject
*VOLTAGE regulators
*FLUORESCEIN
*ALKALINE phosphatase
*ENZYMES
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Language
ISSN
1530-6984
Abstract
We report the enzymatic cleavage of fluorescein diphosphate (FDP) by alkaline phosphatase (AP) confined in the tip of a nanopipet with a volume of approximately 100 attoliters. The amount of enzyme that reversibly adsorbs in the pipet tip was shown to be proportional to the enzyme concentration in the bath. Increasing the voltage applied to the pipet, up to 1.5 V, linearly controls the substrate turnover rate by increasing the flow of substrate out of the pipet. This work opens up possibilities of highly miniaturized and sensitive enzyme assays with high spatial resolution. [ABSTRACT FROM AUTHOR]