부산대학교 도서관

Dear Editor, The exosome, a kind of extracellular vesicle with a diameter in the range of 50-200 nm, has been extensively investigated in treating various skin issues, such as skin aging mitigation and cutaneous wound healing stimulation, and atopic dermatitis (AD).[[1], [3]] Compared to stem cell therapy, advantageous properties, such as a long half-life, small size, favorable penetration, and low immunogenicity, make exosomes more favorable and beneficial.[4] Although exosomes have been extensively investigated for their application in skin damage repair, the only sources shown to be effective are those derived from mesenchymal stem cells (MSCs).[5] However, exosome isolation from MSCs accompanies multiple drawbacks, such as the time and effort required for MSC culture and expansion, the risk of contamination with red blood cells during the isolation process, neovascularization potential, diminished cell viability, and the extreme invasiveness of removing MSCs from bone marrow.[6] Therefore, finding an alternative cell source for exosome isolation has become imperative for the effective application of exosomes in the repair of dysfunctional skin. The absorbance was then measured at 540 nm using a spectrophotometer (Molecular Devices, San Jose, CA, USA). gl Exosome treatment increased the protein levels of skin permeability barrier biomarkers, compared to the control in HaCaT cells. To investigate the effects of DF exosomes on skin permeability barrier protection, we evaluated the expression levels of skin permeability barrier maintenance biomarkers in keratinocytes treated with 1-chloro-2,4-dinitrobenzene (DNCB). Hence, we evaluated the expression levels of skin permeability barrier maintenance biomarkers in keratinocytes treated with DNCB, a skin irritant, and inducer of dermatitis, including AD. [Extracted from the article]