부산대학교 도서관

The halotolerant algae Dunaliella salina produces high levels of carotenoids, antioxidants, and fatty acids, which are required components of aquaculture feed, particularly in brine shrimp rearing. Obtaining high biomass in a low-cost medium is essential in the process of feed production. For future enhancement of Dunaliella biomass production, low-cost media using combinations of commercially available salt and fertilizer was compared to the medium used in current culturing methods. D. salina growth was observed over a range of salinities in commercial fertilizer and quantified using automatic cell counting and UV-vis spectrophotometry. Growth was then observed in an all-commercialized salt and fertilizer medium, followed by a commercialized salt and custom fertilizer medium, and characterized by UV-vis spectrophotometry. D. salina exhibited an optimum growth rate and cell density in 0.25M commercialized salt media with a custom fertilizer mixture. When grown with Miracle-gro fertilizer, growth rates in NaCl and commercialized salt media were equivalent and optimal at 0.50M, but with a lower cell density. The primary results of biomass, cell density, and growth rate indicate that a commercial salt and fertilizer medium shows promising potential for low-cost mass culture of Dunaliella salina in the aquaculture industry. Future work includes lipid and protein content analysis of algae grown in optimized salt and nutrient conditions. [ABSTRACT FROM AUTHOR]