학술논문
On-Chip Immunoassay for Determination of Urinary Albumin.
Document Type
Article
Author
Source
Subject
*IMMUNOASSAY
*ALBUMINS
*MICROFLUIDIC devices
*FLUIDICS
*AGGLUTININS
*DIMETHYLPOLYSILOXANES
*INTEGRATED circuits
*SPECTROMETERS
*STANDARD deviations
*SPECTROPHOTOMETRY
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Language
ISSN
1424-8220
Abstract
An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L-1 (r2 = 0.993), with a detection limit of 0.81 mg L-1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L-1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r2 = 0.995; n = 15). [ABSTRACT FROM AUTHOR]