학술논문
Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor.
Document Type
Article
Source
Subject
*PHOTORECEPTORS
*CELLULAR signal transduction
*STENOTROPHOMONAS maltophilia
*GENETIC mutation
*MUTAGENESIS
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Language
ISSN
0014-5793
Abstract
BLUF domains are flavin-based photoreceptors which receive the blue light signal and are involved in the sensory transduction. We report a short BLUF photoreceptor (SnfB) in Stenotrophomonas sp. We have investigated photodynamic properties of C terminus truncated and several mutated SnfB proteins. Deletion of the extended C-terminal residues alters the thermal recovery kinetics and also affects the integrity of the SnfB protein. Mutagenesis studies demonstrated that the conserved residues within and outside the flavin-binding pocket also regulates the photocycle properties of the protein. These studies suggest that the C-terminal residues outside the BLUF domain can tune the photodynamic properties of the BLUF protein. [ABSTRACT FROM AUTHOR]