학술논문
DNA methylation profile in CpG-depleted regions uncovers a high-risk subtype of early-stage colorectal cancer.
Document Type
Article
Author
Yu, Huichuan; Wang, Xiaolin; Bai, Liangliang; Tang, Guannan; Carter, Kelly T; Cui, Ji; Huang, Pinzhu; Liang, Li; Ding, Yanqing; Cai, Muyan; Huang, Meijin; Liu, Huanliang; Cao, Guangwen; Gallinger, Steven; Pai, Rish K; Buchanan, Daniel D; Win, Aung Ko; Newcomb, Polly A; Wang, Jianping; Grady, William M
Source
Subject
*DNA methylation
*COLORECTAL cancer
*TUMOR markers
*PROGNOSIS
*EPIGENOMICS
*MACHINE learning
*HEREDITARY nonpolyposis colorectal cancer
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Language
ISSN
0027-8874
Abstract
Background The current risk stratification system defined by clinicopathological features does not identify the risk of recurrence in early-stage (stage I-II) colorectal cancer (CRC) with sufficient accuracy. We aimed to investigate whether DNA methylation could serve as a novel biomarker for predicting prognosis in early-stage CRC patients. Methods We analyzed the genome-wide methylation status of CpG loci using Infinium MethylationEPIC array run on primary tumor tissues and normal mucosa of early-stage CRC patients to identify potential methylation markers for prognosis. The machine-learning approach was applied to construct a DNA methylation–based prognostic classifier for early-stage CRC (MePEC) using the 4 gene methylation markers FAT3 , KAZN , TLE4 , and DUSP3. The prognostic value of the classifier was evaluated in 2 independent cohorts (n = 438 and 359, respectively). Results The comprehensive analysis identified an epigenetic subtype with high risk of recurrence based on a group of CpG loci in the CpG-depleted region. In multivariable analysis, the MePEC classifier was independently and statistically significantly associated with time to recurrence in validation cohort 1 (hazard ratio = 2.35, 95% confidence interval = 1.47 to 3.76, P < .001) and cohort 2 (hazard ratio = 3.20, 95% confidence interval = 1.92 to 5.33, P < .001). All results were further confirmed after each cohort was stratified by clinicopathological variables and molecular subtypes. Conclusions We demonstrated the prognostic statistical significance of a DNA methylation profile in the CpG-depleted region, which may serve as a valuable source for tumor biomarkers. MePEC could identify an epigenetic subtype with high risk of recurrence and improve the prognostic accuracy of current clinical variables in early-stage CRC. [ABSTRACT FROM AUTHOR]