부산대학교 도서관

Fast inhibition in the nervous system is commonly mediated by GABAA receptors comprised of 2α/2β/1γ subunits. In contrast, GABAC receptors containing only ρ subunits (ρ1-ρ3) have been predominantly detected in the retina. However, here using reverse transcription-PCR and in situ hybridization we show that mRNA encoding the ρ1 subunit is highly expressed in brainstem neurons. Immunohistochemistry localized the ρ1 subunit to neurons at light and electron microscopic levels, where it was detected at synaptic junctions. Application of the GABAC receptor agonist cis-4-aminocrotonic acid (100-800 µM) requires the ρ1 subunit to elicit responses, which surprisingly are blocked independently by antagonists to GABAA (bicuculline, 10 µM) and GABAC [(1, 2, 5, 6-tetrahydropyridin-4-yl)methylphosphinic acid (TPMPA); 40-160 µM] receptors. Responses to GABAC agonists were also enhanced by the GABAA receptor modulator pentobarbitone (300 µM). Spontaneous and evoked IPSPs were reduced in amplitude but never abolished by TPMPA, but were completely blocked by bicuculline. We therefore tested the hypothesis that GABAA and GABAC subunits formed a heteromeric receptor. Immunohistochemistry indicated that ρ1 and α1 subunits were colocalized at light and electron microscopic levels. Electrophysiology revealed that responses to GABAC receptor agonists were enhanced by the GABAA receptor modulator zolpidem (500 nM), which acts on the α1 subunit when the γ2 subunit is also present. Finally, coimmunoprecipitation indicated that the ρ1 subunit formed complexes that also contained α1 and γ2 subunits. Taken together these separate lines of evidence suggest that the effects of GABA in central neurons can be mediated by heteromeric complexes of... [ABSTRACT FROM AUTHOR]