부산대학교 도서관

Besides metabolic pathways and regulatory networks, transport systems are also pivotal for cellular metabolism and hyperproduction of biochemicals using microbial cell factories. The identification and characterization of transporters are therefore of great significance for the understanding and engineering of transport reactions. Herein, a novel L-glutamate exporter, MscCG2, which exists extensively in Corynebacterium glutamicum strains but is distinct from the only known L-glutamate exporter, MscCG, was discovered in an industrial L-glutamate-producing C. glutamicum strain. MscCG2 was predicted to possess three transmembrane helices in the N-terminal region and located in the cytoplasmic membrane, which are typical structural characteristics of the mechanosensitive channel of small conductance. MscCG2 has a low amino acid sequence identity (23%) to MscCG and evolved separately from MscCG with four transmembrane helices. Despite the considerable differences between MscCG2 and MscCG in sequence and structure, gene deletion and complementation confirmed that MscCG2 also functioned as an L-glutamate exporter and an osmotic safety valve in C. glutamicum. Besides, transcriptional analysis showed that MscCG2 and MscCG genes were transcribed in similar patterns and not induced by L-glutamate-producing conditions. It was also demonstrated that MscCG2- mediated L-glutamate excretion was activated by biotin limitation or penicillin treatment and that constitutive L-glutamate excretion was triggered by a gain-offunction mutation of MscCG2 (A151V). Discovery of MscCG2 will enrich the understanding of bacterial amino acid transport and provide additional targets for exporter engineering. [ABSTRACT FROM AUTHOR]