학술논문
Circadian variation affects the biology and digestive profiles of a nocturnal insect Spodoptera litura (Insecta: Lepidoptera).
Document Type
Article
Source
Subject
*SPODOPTERA littoralis
*CIRCADIAN rhythms
*LACTATE dehydrogenase
*EFFECT of light on insects
*POLYPEPTIDES
*DIGESTIVE enzymes
*PHOTOPERIODISM
*INSECTS
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Language
ISSN
0929-1016
Abstract
The present study was conducted to decipher the impact of circadian rhythm on digestive enzymes ofSpodoptera lituraunder three photoperiods (12L:12D, 0L:24D, and 24L:0D). Longer life cycle, higher developmental traits and significant food utilizing capability were observed in dark conditions (DD), while there was no effect on survival. The activity of lactate dehydrogenase (LDH), α and β-glucosidase depended on complete absence of light (DD) while LL had a significant effect on protease activity. The presence of polypeptides (35, 60 kDa) and lower protease inhibition by PMSF in 0L:24D, and 24L:0D indicated that serine proteases (trypsin) were the main proteases in larval midgut. Overall, zymography profiles suggested that circadian variation, particularly dark period influenced theS. lituradevelopment due to fluctuations in the midgut enzymes via food utilization. Although the effect of photoperiod on digestive processes of insects is still unclear, dark regime may underlie the midgut digestive enzymes inS. lituralarvae. [ABSTRACT FROM AUTHOR]