부산대학교 도서관

The aim of this investigation was to evaluate the shielding character of double coated beads of alginate-chitosan- Eudragit S100 nanoparticles attained from encapsulation of Lactobacillus acidophilus. After activation of starter culture of Lactobacillus acidophilus in MRS-broth, centrifuge (5000 rpm for 10 min) was employed to purify bacteria. The examination of beads strength was performed for 12 h and the assessment of viability of encapsulated bacteria was done for 120 min inside phosphate buffer, hydrochloric acid, and digestive powder solution. Pour plate process with MRS-Salicin-agar, and incubation at 37 °C for 48 h was used for cultivation. Recorded data were analyzed via an independent t-test. The results showed that viability of microencapsulated bacteria in both condition with and without tensions, is significantly further than free ones (p<0/05). Microencapsulation by means of alginate-chitosan- Eudragit S100 nanoparticles plays a major role in enhancing the amount of Lactobacillus acidophilus survival. [ABSTRACT FROM AUTHOR]