부산대학교 도서관

This study aims to emphasize the bioactive ingredients, antioxidant, anticancer, and anti-influenza (H5N1) effects of different extracts of Urtica urens L.. Three different extracts were prepared successively based on polarity yielding diethyl ether (DE), ethyl acetate (EA), and methanol (ME) extracts. GC-MS analysis revealed cyclopentasiloxane-decamethyl, quinine-1,1'-dioxide, and carotene as the prominent compounds in DE extract. The three extracts contain adequate amounts of total phenolics, total flavonoids, and total tannins and the superior amounts were detected in EA extract. Hesperidin, apigenin-6-arabinose-8-galactose, pyrogallol, and ferulic acid were the predominant individual phenolics identified and quantified in EA and ME extracts by HPLC, with a higher concentration in EA than in ME. The EA extract exhibited the highest antioxidant activity with IC50 306.15±2.27 and 103.32±0.34 µg/ml for DPPH and ABTS assays, respectively and EC50 352.50±2.50 µg/ml for reducing power assay. The DE and EA extracts have an efficient cytotoxicity effect against breast cancer cells (MCF-7) with IC50 values 28.9 and 48.58 µg/ml and colon cancer cells (HCT116) with IC50 values 39.81 and 41.21µg/ml, respectively. This is the first report regarding the antiviral effect of DE and ME extracts of U. urens against the avian influenza H5N1 virus. The DE exhibited the highest antiviral activity through the inhibition of H5N1 propagation with IC50 value 125 µg/ml. The results of the mode of action against H5N1 virus showed the potent virucidal effect of DE extract with 80% inhibition of viral propagation. The DE extract showed a limited effect on H5N1 viral replication and viral adsorption process. The DE extract of Urtica urens exhibited the highest anticancer and anti-influenza (H5N1) effects besides adequate antioxidant activity and is recommended for future use in the pharmaceutical applications for H5N1 and cancer (breast and colon) treatment. [ABSTRACT FROM AUTHOR]