부산대학교 도서관

Graphical abstract Schematic illustration of graphene cellulose (G-C) paper fabrication, application for 3D multilayered laminate cell-laden constructs, and ensuing "origami-inspired" sculpting for 3D tissue engineering and regeneration. Highlights • Efficient method for graphene-cellulose (G-C) paper fabrication. • G-C paper is electroconductive, mechanically robust, flexible and biocompatible. • G-C paper can be employed for 2D or 3D stem cell support and differentiation. • G-C papers with cell-laden alginate can be stacked as 3D multilayered constructs. • Multilayered cell-laden constructs can be further configured by folding or rolling. Abstract Graphene-based materials represent advanced platforms for tissue engineering and implantable medical devices. From a clinical standpoint, it is essential that these materials are produced using non-toxic and non-hazardous methods, and have predictable properties and reliable performance under variable physiological conditions; especially when used with a cellular component. Here we describe such a biomaterial, namely smart graphene-cellulose (G-C) paper, and its suitability for traditional planar two-dimensional (2D) or three-dimensional (3D) human cell support, verified by adipose-derived stem cell (ADSC) culture and osteogenic differentiation. G-C paper is prepared using commercially available cellulose tissue paper as a substrate that is coated by immersion-deposition with graphene oxide (GO) followed by reduction to reduced graphene oxide (RGO) without the use of toxic organic solvents. The fabrication process is amenable to large scale production and the resultant papers have low electrical resistivity (up to ∼300 Ω/sq). Importantly, G-C papers can be configured to 3D constructs by lamination with alginate and further modified by folding and rolling for 3D "origami-inspired" cell-laden structures. [ABSTRACT FROM AUTHOR]