학술논문
Development of an immunochromatographic strip assay based on a monoclonal antibody for detection of cimaterol.
Document Type
Article
Author
Source
Subject
*ENZYME-linked immunosorbent assay
*ALPHA fetoproteins
*MONOCLONAL antibodies
*DETECTION limit
*URINE
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Language
ISSN
0954-0105
Abstract
An anti-cimaterol (cima) monoclonal antibody, 2H8, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip assay for the detection of cima, a newly described β-agonist. The half-maximal inhibitory concentration of the antibody was 1.56 ng/mL, and no cross-reactivity with other β-adrenergic agonists was observed. The antibody's limit of detection was 0.416 ng/mL, and its linear range of detection was 0.416–5.868 ng/mL. The recovery rates of cima in swine urine ranged from 86% to 92.5%, and the test strip had a cut-off value of 10 ng/mL. Both ic-ELISA and the developed strip assay are effective when detecting cima in swine urine. [ABSTRACT FROM AUTHOR]