학술논문

Isolation and characterisation of cadmium-resistant bacteria from an industrially polluted coastal ecosystem on the southeast coast of India.
Document Type
Article
Source
Chemistry & Ecology. Oct2014, Vol. 30 Issue 7, p622-635. 14p.
Subject
*ECOSYSTEMS
*PHYSIOLOGICAL effects of cadmium
*NUCLEOTIDE sequence
*RIBOSOMAL RNA
Language
ISSN
0275-7540
Abstract
A total of 35 bacterial strains were isolated from the industrially polluted Cuddalore coast, on the southeast coast of India. Of these, 17 strains were cadmium resistant and the remainder were sensitive. Six strains (C-1, C-8, C-10, C-12, C-14 and N-1) were selected based on high levels of cadmium tolerance (>150 mg L−1) and were termed highly cadmium-resistant bacteria (HCRB). These HCRB were identified on the basis of morphological, biochemical and partial sequencing of their 16S rRNA genes. The antibiotic-susceptibility patterns and minimum inhibitory concentrations (MIC) of different metals (Cu2+, Pb2+and Zn2+) against each HCRB were determined. Among the isolates, C-14 showed high degrees of metal and antibiotic resistance compared with other HCRB. Growth rates of HCRB at two different Cd2+concentrations (50 and 100 mg L−1) and under different metal conditions (Cd2+, Cu2+and Pb2+) were also investigated. HCRB growth rates were lower in the metal-treated condition than in the untreated condition. Isolates C-14 and N-1 removed>80% of Cd2+from cadmium-treated broth. However, isolate C-14 removed 92.3% of Cd2+compared with 86.5% for isolate N-1. Bacteria showing residual growth rates under metal stress conditions might be useful in metal removal applications under growing conditions. [ABSTRACT FROM PUBLISHER]