학술논문
Oxidation induces autonomous activation of protein kinase C Apl I, but not protein kinase C Apl II in homogenates of Aplysia neurons
Document Type
Article
Author
Source
Subject
*NEURONS
*APLYSIA
*PROTEIN kinase C
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Language
ISSN
0304-3940
Abstract
The Ca2+-independent protein kinase C (PKC) Apl II, but not the Ca2+-activated PKC Apl I, becomes autonomously active during intermediate periods of facilitation in Aplysia neurons. We examined the ability of superoxide formed by the enzymatic reaction of xanthine with xanthine oxidase (X/XO) to induce autonomous activity of PKCs in Aplysia. X/XO stimulated autonomous PKC activity in Aplysia nervous system homogenates, but this activity resulted solely from activation of PKC Apl I. PKC Apl I is also more sensitive to activation by X/XO when expressed in insect cells. Our results suggest that oxidation can autonomously activate PKC Apl I in the Aplysia nervous system, but that the activation of PKC Apl II during synaptic facilitation is not due to oxidation of the enzyme. [Copyright &y& Elsevier]