부산대학교 도서관

Objective: To examine the extent to which rapid thromboelastography (r‐TEG) could decrease the testing time in comparison with that required for kaolin‐activated thromboelastography (TEG), and to compare 2 types of blood samples (ie, native and citrated whole blood [WB]), for determining r‐TEG values in healthy dogs. Design: Prospective observational study. Setting: University teaching hospital. Animals: Sixteen healthy Beagles. Interventions: Kaolin‐activated TEG test using citrated WB samples and r‐TEG test using native and citrated WB samples were performed in 16 dogs. At 60 minutes after the initial blood sampling, further samples were collected from a subset of 6 dogs in the same manner to evaluate intraindividual repeatability of r‐TEG. Measurements and Main Results: The mean time to maximum amplitude (MA) for r‐TEG with native and citrated WB samples was recorded as 1313.9 ± 250.9 seconds and 1351.3 ± 264.6 seconds (mean ± SD), respectively, and 1779.9 ± 197.0 seconds for kaolin‐activated TEG. Coefficients of variation with native and citrated WB samples for r‐TEG values, TEG‐activated clotting time, clot formation time, α angle, and MA, were determined to be 13.4% versus 18.8%, 11.1% versus 16.6%, 4.2% versus 5.1%, and 10.0% versus 10.0%, respectively. Intraindividual variations were lower for native WB samples than for citrated WB samples. Conclusions: The r‐TEG test significantly decreased the mean time to MA compared with the kaolin‐activated TEG test. In addition, native WB samples showed lower coefficients of variation and intraindividual variation than citrated WB samples in r‐TEG analysis; this suggests that native WB samples can provide more consistent results. Therefore, the r‐TEG method using native WB samples is recommended for assessment of dogs' hemostatic status when an early diagnosis is required. [ABSTRACT FROM AUTHOR]