학술논문
DEVELOPMENT AND CHARACTERIZATION OF MICROSATELLITE LOCI IN PERICOPSIS ELATA (FABACEAE) USING A COST-EFFICIENT APPROACH.
Document Type
Article
Source
Subject
*MICROSATELLITE repeats
*REPEATED sequence (Genetics)
*LEGUMES
*TREES
*BOTANY
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Language
ISSN
0002-9122
Abstract
• Premise of the study: Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. • Methods and Results: Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like labeling in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata , and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus. • Conclusions: These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa. [ABSTRACT FROM AUTHOR]