학술논문
Plasmodium vivax: Comparison of the immune responses between oral and parenteral immunization of rPv54 in BALB/c mice
Document Type
Article
Author
Source
Subject
*PLASMODIUM vivax
*IMMUNIZATION
*IMMUNOREGULATION
*LABORATORY mice
*MICROBIAL proteins
*ORAL vaccines
*GEL electrophoresis
*COMPARATIVE studies
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Language
ISSN
0014-4894
Abstract
Abstract: The merozoite surface protein-1 (MSP-1) from Plasmodium vivax was evaluated as an oral vaccine candidate by cloning and expressing the interspecies conserved block 10 (ICB10) of the MSP-1 from a Korean isolate in Escherichia coli. The expressed fusion protein contained ICB10 and a maltose-binding protein (MBP), rPv54, has a molecular weight of approximately 54kDa as determined by SDS–PAGE analysis. IgG against rPv54 was successfully produced in BALB/c mice by oral immunization and sustained for more than 4months. IgG2b was dominantly produced in both oral and parenteral immunizations. The rPv54 increased the frequency of NK, NKT, CD4+ T, CD8+ T, and B cells in both immunizations. IL-5 and TNF-α were increased in both significantly. In conclusion, rPv54 might be a valuable potential vaccine candidate for the oral and parenteral immunization against vivax malaria. [ABSTRACT FROM AUTHOR]