부산대학교 도서관

Aim: The study assessed mRNA expression of podocyte-associated molecules in urinary sediments of patients with type 2 diabetes mellitus (DM) in relation to urinary podocytes, biomarκers of podocyte injury and of proximal tubule (PT) dysfunction. Methods: A total of 76 patients with type 2 DM and 20 healthy subjects were enrolled in a cross-sectional study, and assessed concerning urinary podocytes, urinary mRNA of podocyte-associated genes, urinary biomarκers of podocyte damage and of PT dysfunction. Results: We found significant differences between urinary mRNA of podocyte-associated molecules in relation with albuminuria stage. In multivariable regression analysis, urinary mRNA of nephrin, podocin, alpha-actinin-4, CD2-associated protein, glomerular epithelial protein 1 (GLEPP1), ADAM 10, and NFκB correlated directly with urinary podocytes, albuminuria, urinary alpha1-microglobulin, urinary κidney-injury molecule-1, nephrinuria, urinary vascular endothelial growth factor, urinary advanced glycation end-products (AGE), and indirectly with eGFR (p < 0.0001, R2= 0.808;p <0.0001, R2=0.825; p < 0.0001, R2= 0.805; p &360; 0.0001, R2= 0.663; p < 0.0001, R2= 0.726; p & 0.0001, R2= 0.720;p < 0.0001, R2= 0.724). Conclusions: In patients with type 2 DM there is an association between urinary mRNA of podocyte-associatedmolecules, biomarκers of podocyte damage, and of PT dysfunction. GLEPP1, ADAM10, and NFκB may be consideredadditional candidate molecules indicative of early diabetic nephropathy. AGE could be involved in this association. [ABSTRACT FROM AUTHOR]