학술논문

Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination.
Document Type
Article
Source
Archives of Virology. Feb2015, Vol. 160 Issue 2, p543-547. 5p.
Subject
*TOMATO ringspot virus
*RASPBERRIES
*NUCLEOTIDE sequence
*GENETIC recombination
*RNA viruses
REPRODUCTIVE isolation
Language
ISSN
0304-8608
Abstract
The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5′-terminal 900 nucleotides and 3′ untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3′ UTR and 5′-terminal region of RNA1. [ABSTRACT FROM AUTHOR]