학술논문
Switching on RNA Silencing Suppressor Activity by Restoring Argonaute Binding to a Viral Protein.
Document Type
Article
Source
Subject
*RNA
*GENE silencing
*SUPPRESSOR mutation
*VIRAL mutation
*ARGONAUTE proteins
*PROTEIN binding
*VIRAL proteins
*SWEET potato diseases & pests
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Language
ISSN
0022-538X
Abstract
We found that Sweet potato feathery mottle virus (SPFMV) P1, a close homologue of Sweet potato mild mottle virus P1, did not have any silencing suppressor activity. Remodeling the Argonaute (AGO) binding domain of SPFMV P1 by the introduction of two additional WG/GW motifs converted it to a silencing suppressor with AGO binding capacity. To our knowledge, this is the first instance of the transformation of a viral protein of unknown function to a functional silencing suppressor. [ABSTRACT FROM AUTHOR]