학술논문
Isolation and Characterization of Human Lung Lymphatic Endothelial Cells.
Document Type
Article
Author
Source
Subject
*LYMPHATIC physiology
*ENDOTHELIUM
*CANCER patients
*CELL physiology
*CELL separation
*ELECTRON microscopy
*TISSUE fixation (Histology)
*FLOW cytometry
*IMMUNOHISTOCHEMISTRY
*LUNGS
*LUNG tumors
*RESEARCH methodology
*RESEARCH funding
*TISSUE culture
*TRANSFERASES
*IN vitro studies
*ANATOMY
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
Language
ISSN
2314-6133
Abstract
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy. [ABSTRACT FROM AUTHOR]