학술논문
Lack of effect of Theiler’s murine encephalomyelitis virus infection on system xc−.
Document Type
Article
Author
Source
Subject
*THEILER'S murine encephalomyelitis virus
*MULTIPLE sclerosis treatment
*MACROPHAGES
*PROTEIN expression
*GLUTAMATE transporters
*DEMYELINATION
*CONTROL groups
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Language
ISSN
0304-3940
Abstract
Changes in the expression of x CT, the specific subunit of system x c − or the cystine/glutamate antiporter, have been associated with several neurological disorders and system x c − was recently proposed as a potential target for the development of new treatment strategies for multiple sclerosis (MS). In this study we used Theiler's murine encephalomyelitis virus (TMEV) infection, both in vitro and in vivo , as a model to further evaluate the involvement of system x c − in MS. Protein levels of x CT, as well as activity of system x c − were unaffected in RAW264.7 macrophages after infection with the demyelinating DA strain of TMEV. Also, protein expression of x CT remained stable in spinal cord and brain of FVB mice 1–2 and 6 weeks after intracranial injection of the DA strain of TMEV. These results demonstrate that TMEV infection of macrophages or FVB mice has no effect on system x c − and as such cannot be used as a model to study the involvement of system x c − in MS. [ABSTRACT FROM AUTHOR]