학술논문
Metabolic response patterns of nucleotides in B-cell chronic lymphocytic leukaemias to cladribine, fludarabine and deoxycoformycin
Document Type
Article
Source
Subject
*NUCLEOTIDES
*FLUDARABINE
*ANTINEOPLASTIC agents
*NUCLEIC acids
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Language
ISSN
0145-2126
Abstract
Nucleotides (NTPs and dNTPs) have been measured in patient-derived chronic lymphocytic leukaemia (CLL) cells stimulated with TPA plus ionomycin and then exposed to cladribine, fludarabine or deoxycoformycin (1 μM, 16 h). dNTPs were not measurable in 108 unstimulated CLL cells which are quiescent. Time-dependent changes in nucleotides in CLL cells showed that the mechanism of action changed as the drug triphosphate accumulated. dCf induced substantial accumulation of dATP in CLL cells in culture, and similar levels of dATP in the same patient during subsequent treatment with dCf. Determination of “metabolic response patterns” of nucleotides in CLL cells treated with drugs might distinguish patients with susceptible and refractory CLL prior to chemotherapy. [Copyright &y& Elsevier]