부산대학교 도서관

Proteases have essential qualities which have attained the attention of researches from different domains including biotechnology and have thus become important group of enzymes both for industrial use and academic research purpose. Bacillus sp. is commonly used for isolation and extraction of protease enzyme. This study reports the kinetics of proteases from Bacillus sp. Results showed that maximum/optimum activity was recorded at 60°C temperature, 7.5 pH and 48 hours of continuous shaking at 220 rpm. The effects of different metal-ions on activity of proteases was also assessed. Lower value of KM=0.2 obtained from Lineweaver- Burk plot for the substrate demonstrated good association/relationship between substrate and protease enzyme. Dependence of the enzyme activity on the concentration of substrate revealed a good agreement between the experimental and theoretical values. PMSF inhibition proved that the proteases produced were serine proteases. Overall, the unique characters of proteases isolated from the Bacillus sp. suggested this enzyme for having remarkable potential for use in different industrial applications. [ABSTRACT FROM AUTHOR]