학술논문
Clonality analysis of hematopoietic cell lineages in acute myeloid leukemia and translocation (8;21): only myeloid cells are part of the malignant clone.
Document Type
Journal Article
Source
Subject
*MYELOID leukemia
*FLUORESCENCE in situ hybridization
*BONE marrow
*CELL differentiation
*CELLS
*CHROMOSOME abnormalities
*CHROMOSOMES
*COMPARATIVE studies
*HEMATOPOIETIC stem cells
*RESEARCH methodology
*MEDICAL cooperation
*RESEARCH
*STEM cells
*EVALUATION research
*ACUTE myeloid leukemia
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Language
ISSN
0887-6924
Abstract
Bone marrow from six patients with acute myeloid leukemia (AML) and t(8;21) (q22;q22) or a variant t(8;13;21) was studied by simultaneous analysis of cell morphology and karyotype. Combination of May-Grünwald-Giemsa (MGG) and fluorescence in situ hybridization (FISH) using probes specific for the breakpoint regions of chromosome 8 and 21 allowed us to establish the extent of cell-lineage involvement of the translocation. The translocation was found in all myeloid blasts and in high percentages of the more mature neutrophilic cells. In one patient we could demonstrate the translocation in the eosinophils as well. Erythroblasts and lymphocytes did not show the t(8;21) abnormality. These results indicate that the t(8;21) in AML is restricted to the myeloid (granulocytic) lineage. [ABSTRACT FROM AUTHOR]