학술논문
ANALYSIS OF HISTAMINE AS INDICATOR OF SHELF-LIFE IN SEAFOOD.
Document Type
Article
Author
Source
Subject
*HISTAMINE
*SHELF-life dating of food
*SEAFOOD
*HIGH performance liquid chromatography
*BIOGENIC amines
*THERMOSTAT
*DERIVATIZATION
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Language
ISSN
1120-1770
Abstract
In the present study, a reversed high performance liquid chromatographic method with diode array detection was developed to analyze histamine in sea food samples. The separation was performed on a Kromasil ODS (C18) (150x3.20 mm, 5 µm) column thermostated at 25°C and using a gradient of Milli-Q water and methanol as mobile phase. The biogenic amine was extracted from seafood samples by using 0.1 N HC1 and derivatized with dansyl chloride. Histamine contents ranged from 1.0 to 2.5mg/kg. [ABSTRACT FROM AUTHOR]