학술논문
Uncharged Gemini-Amphiphiles as Components of Cationic Liposomes for Delivery of Nucleic Acids.
Document Type
Article
Author
Source
Subject
*NUCLEIC acids
*EUKARYOTIC cells
*CATIONIC lipids
*AMPHIPHILES
*GENE transfection
*DNA
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Language
ISSN
1068-1620
Abstract
New uncharged gemini-amphiphiles have been synthesized. A series of cationic liposomes based on the polycationic amphiphile 1,26-bis(cholest-5-en-3β-yloxycarbonylamino)-7,11,16,20-tetraazahexacosan tetrahydrochloride (2X3), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and uncharged gemini-amphiphiles that contain short hydrophobic and long hydrophilic spacers have been prepared. The physicochemical characteristics, cytotoxicity, and transfection activity of cationic liposomes have been studied. Cationic liposomes that include 10% (mol) of a uncharged gemini-amphiphile with the oligooxymethylene spacer have formed small complexes with plasmid DNA and efficiently delivered it into eukaryotic cells. [ABSTRACT FROM AUTHOR]