부산대학교 도서관

While Pichia pastoris has been developed into a versatile recombinant protein expression system, there are only few studies that have investigated the efficacious use of this yeast with human cells. In this study, we demonstrated that P. pastoris can be cultured under mammalian cell culture conditions and co-cultured with human endothelial cells. Co-cultures did not affect endothelial cell morphology or viability. Additionally, P. pastoris was induced to express enhanced green fluorescence protein when co-cultured with human endothelial cell line EA.hy926 under mammalian cell culture conditions. Our study provides data to support the use of P. pastoris as a vehicle for direct delivery of recombinant proteins to mammalian cells during co-culture. • Pichia pastoris grows slower with mammalian culture media and conditions • Endothelial cells co-cultured with P. pastoris • P. pastoris does not affect mammalian cell NF-κB pathways • P. pastoris express EGFP when cultured with endothelial cells [ABSTRACT FROM AUTHOR]