학술논문
Thermal denaturation and autolysis profiles of myofibrillar proteins of mantle muscle of jumbo squid Docidicus gigas.
Document Type
Article
Source
Subject
*ADENOSINE triphosphatase
*AUTOLYSIS
*CALCIUM ions
*SQUIDS
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Language
ISSN
0919-9268
Abstract
ABSTRACT: Jumbo squid was very similar to Japanese common squid in terms of myofibrillar Ca2+ -, Mg2+ - and K+ (EDTA)-ATPase activities. Myofibrils of jumbo squid were significantly stabilized upon addition of Ca2+ and destabilized by increasing KCl concentration for heating. Incubation of muscle homogenate of jumbo squid induced a selective cleavage of myosin into two major fragments and the cleavage was inhibited by EDTA. Autolysis was prominent at and above 0.3 M NaCl where myosin filaments dissolve. The enzyme involved in the autolysis was proved to be unstable showing maximal autolysis rate at 25°C. Washing the homogenate partially reduced the autolysis activity. [ABSTRACT FROM AUTHOR]