학술논문
A Reappraisal, Based on [sup 31]P NMR, of the Direct Coordination of a Metal Ion with the Phosphoryl Oxygen at the Cleavage Site of a Hammerhead Ribozyme.
Document Type
Article
Author
Source
Subject
*BINDING sites
*SCISSION (Chemistry)
*CATALYTIC RNA
*METAL ions
*
*
*
Language
ISSN
0002-7863
Abstract
Determines whether or not a metal ion would bind with the phosphoryl oxygen at the cleavage site of a hammerhead ribozyme with the use of nuclear magnetic resonance (NMR) spectroscopy. Extent of the perturbations of NMR signals from phosphorothioates; Absence of any change in NMR signals at the cleavage site upon the addition of excess metal ions.