학술논문
Sortilin 1 regulates hepatocellular carcinoma progression by activating the PI3K/AKT signaling.
Document Type
Article
Source
Subject
*THERAPEUTIC use of antineoplastic agents
*DISEASE progression
*IN vitro studies
*FLOW cytometry
*WOUND healing
*IN vivo studies
*ONCOGENES
*WESTERN immunoblotting
*CANCER invasiveness
*APOPTOSIS
*METASTASIS
*CELLULAR signal transduction
*TREATMENT effectiveness
*CELL survival
*CELL motility
*CELL proliferation
*TUMOR markers
*CELL lines
*HEPATOCELLULAR carcinoma
*ENZYME inhibitors
*EVALUATION
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Language
ISSN
0960-3271
Abstract
Background: Sortilin 1 (SORT1) has been reported as an oncogene in several human tumors. Nonetheless, the biological functions of SORT1 in hepatocellular carcinoma (HCC) remain poorly understood. Methods: Western blotting was employed for the determination of protein expression. Hepatocellular carcinoma cell viability, apoptosis, migration, and invasion were measured via CCK-8, flow cytometry, wound healing, and Transwell assays. Results: Sortilin 1 was upregulated in HCC and closely associated with unsatisfactory outcomes of HCC patients. Furthermore, in vitro and in vivo assays revealed that SORT1 knockdown significantly diminished HCC cell proliferation and metastasis but accelerated HCC cell apoptosis; moreover, SORT1 depletion also restrained the growth of xenografted HCC tumors. Mechanistically, SORT1 activated PI3K/AKT signaling in HCC cells, thereby promoting the malignant behaviors of HCC cells. Conclusion: This study demonstrated that SORT1 might promote HCC progression by activating PI3K/AKT signaling, indicating that SORT1 might be a promising target and biomarker for HCC treatment and prognosis. [ABSTRACT FROM AUTHOR]