학술논문
Application of fluorescence micro-optical sectioning tomography in the cerebrovasculature and applicable vascular labeling methods.
Document Type
Article
Author
Source
Subject
*FLUORESCENCE
*TOMOGRAPHY
*THREE-dimensional imaging
*TECHNICAL drawing
*HIGH resolution imaging
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Language
ISSN
1863-2653
Abstract
Fluorescence micro-optical sectioning tomography (fMOST) is a three-dimensional (3d) imaging method at the mesoscopic level. The whole-brain of mice can be imaged at a high resolution of 0.32 × 0.32 × 1.00 μm3. It is useful for revealing the fine morphology of intact organ tissue, even for positioning the single vessel connected with a complicated vascular network across different brain regions in the whole mouse brain. Featuring its 3d visualization of whole-brain cross-scale connections, fMOST has a vast potential to decipher brain function and diseases. This article begins with the background of fMOST technology including a widespread 3D imaging methods comparison and the basic technical principal illustration, followed by the application of fMOST in cerebrovascular research and relevant vascular labeling techniques applicable to different scenarios. [ABSTRACT FROM AUTHOR]